Boosting PCR accuracy – tips for maximizing amplification fidelity
In this Mol Bio Minutes episode, Laurynas Alijošius shares a personal story that every molecular biologist can relate to—running PCR, cloning, and sequencing, only to discover frustrating errors in the DNA. This episode dives deep into PCR accuracy and why it matters for everything from sequencing to cloning and long-read library prep. Laurynas breaks down the major contributors to PCR error, including the fidelity of DNA polymerase, primer design flaws, template impurities, and suboptimal cycling conditions. He then offers a range of solutions—like switching to high-fidelity enzymes, using ready-to-go master mixes, and optimizing magnesium ion concentrations. He also explains how reducing cycle numbers and fine-tuning annealing temperatures can minimize unwanted amplification and ensure more reliable data.Whether you're troubleshooting or proactively optimizing your workflow, this episode is packed with tips and tools to help you increase PCR accuracy, reduce costs, and save time. Episode notes contain links to enzyme comparisons, primer design tools, and cycling guides to help you PCR with precision.Helpful resource links mentioned in this episode:Thermo Scientific web tools for primer design and analysis, and moreThe PCR Learning Center with lots of helpful tips and informationLearn more about PCR reagents and enzymesBrowse and purchase PCR enzymes and master mixesAccess the PCR troubleshooting guideDownload the molecular biology handbook
Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague. Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology. For Research Use Only. Not for use in diagnostic procedures.
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18:41
Multiomics, systems biology, and the future of genomic research
In this episode of Speaking of Mol Bio, Dr. Cath Moore of the Australian Genome Research Facility (AGRF) discusses how molecular biology technologies are helping to shape Australia’s scientific landscape—from clinical genomics and conservation to bioremediation and agriculture. With over 20 years of experience in both academia and industry, Dr. Moore reflects on the remarkable evolution of genomic tools, from Sanger sequencing to high-resolution spatial multiomics.She unpacks AGRF’s mission to democratize access to emerging technologies and highlights its role as an early adopter of platforms that help scientists translate academic research into real-world impact. Topics include non-mass spec proteomics, mine site rehabilitation through soil microbiome analysis, and the role of systems biology in modern science.Dr. Moore also discusses the importance of community education and literacy around genomics, emphasizing how public understanding is key to the safe adoption of emerging technologies like synthetic biology. Finally, she shares career insights and advice for aspiring scientists: stay curious, stay broad, and don’t be afraid to pivot when your work no longer brings joy.
Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague. Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology. For Research Use Only. Not for use in diagnostic procedures.
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33:16
Fast PCR fundamentals – from instruments to enzymes
In this Mol Bio Minutes episode, Laurynas Alijošius breaks down how to run fast PCR to save time and increase lab efficiency. He explains how to choose the right thermal cycler with fast ramp speeds, select low-volume and thin-walled PCR plastics, and use engineered DNA polymerases that offer rapid elongation and hot-start capability. Laurynas also covers practical tips for optimizing reaction components, shortening cycling protocols, and reducing waste. Whether you’re aiming to finish your experiment before dinner or streamline your workflow long-term, this episode delivers everything you need to master the art of fast PCR.Helpful resource links mentioned in this episode:Molecular biology handbook – An extensive resource for all things molecular biologyApplied Biosystems thermal cyclers – Including those that support fast PCRPCR consumables – Four key attributes to considerPCR plastics selection tool – Find the right plastics for your instrument and fast PCRDNA polymerases – Four key characteristics to know and considerPCR setup optimization – Six critical things to optimize for optimal PCR resultsEnzymes and master mixes – Get the right reagents to drive your PCR reactionCycling optimization – Instrument considerations for PCR success
Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague. Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology. For Research Use Only. Not for use in diagnostic procedures.
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16:40
The hidden language of RNA – how epigenetics is shaping medicine
This episode teaches that innovation is born at the intersection of curiosity and persistence. Dr. Gudrun Stengel, co-founder and CEO of Alida Biosciences, reveals how her startup is transforming the landscape of RNA research through a novel technology called proximity barcoding. Dr. Stengel’s story exemplifies the power of entrepreneurial spirit in driving scientific discovery, offering a glimpse into how one idea can reshape an entire field.At Alida Biosciences, Dr. Stengel and her team are pioneering new tools for detecting RNA modifications, a largely unexplored realm of epigenetics. Using their proximity barcoding platform, researchers can read multiple RNA modifications simultaneously, uncovering potential biomarkers and therapeutic targets for diseases like cancer, Alzheimer’s, and diabetes. This technology bridges a critical gap in multiomics, allowing scientists to dive deeper into how epigenetic changes influence gene expression and cellular behavior.Beyond the lab, Dr. Stengel shares her experience as a first-time founder, balancing scientific rigor with startup life. From managing a team to fundraising, her journey underscores the importance of resilience, optimism, and maintaining a long-term vision. She also offers valuable advice for budding scientists, encouraging them to embrace challenges and remain persistent in the face of setbacks.
Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague. Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology. For Research Use Only. Not for use in diagnostic procedures.
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31:51
Sustainability made simple – greener choices in molecular biology
Sustainability in the lab isn't just a trend—it's a responsibility. In this quick but powerful Mol Bio Minutes episode, sustainability expert Sune Lund Sporring shares actionable tips to reduce energy use, manage lab waste, and transition to greener materials like Thermo Fisher’s Sustain Series bio-based plastics.Discover the difference between bio-based and biodegradable, why second-generation feedstocks are a game-changer, and how to make low-impact changes without compromising performance. Learn how small shifts—like using aluminum beads instead of water in a bath or switching to carbon-reduced plastics—can significantly cut your lab’s carbon footprint. And remember: if greener options aren’t available, your demand can help shape the market.Helpful resource links mentioned in this episode:My Green Lab – Facts and resources about lab sustainabilityEnergy use of fume hoods – Energy use and savings ideas for fume hoodsGreener by Design – Thermo Fisher’s approach to green solutionsLab Armor™ Beads – A sustainable option to replace water in water bathsInstrument trade up program – trade in your equipment to be green and give it a second lifeLab plastic waste – Stats about plastic waste in labsDoing something about it – Thermo Fisher’s lower carbon plastics solutionSustain Series PCR plastics – Lower carbon footprint, without workflow interruptionReasons to believe – Fact sheet about Sustain Series PCR plastics
Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague. Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology. For Research Use Only. Not for use in diagnostic procedures.
Speaking of Mol Bio, a podcast series from Thermo Fisher Scientific, discusses trending applications in science and the molecular biology aspects of those applications. Our host delves in to deep discussion with CEOs, R&D scientists, researchers, and key opinion leaders across the globe. Speaking of Mol Bio helps scientific curious people - from all scientific and non-scientific backgrounds - understand how modern molecular biology applications can help push the boundaries in medicine, science, drug discovery, and in the cure and treatment of diseases.
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